The rabbit Anti-5-hmC Polyclonal Antibody can robustly distinguish between hydroxymethylated DNA and methylated or unmodified DNA with limited to no cross-reactivity. The antibody has been validated in ELISA and immunoprecipitation-based enrichment assays, and is suitable for use in further applications including immunohistochemical labeling and chromatographic blotting.Low cross-reactivity with cytosine and 5-methylcytosine versus other available antibodies. Can be used with a variety of genomic DNA sources. High sensitivity to low masses of 5-hydroxymethylcytosine DNA.Storage Temperature: -20CStorage Conditions: FreezerBuffer Solution: Antibody is provided in PBS (Phosphate Buffered Saline, pH 7.4) with 0.05% Sodium azide as a preservative. Isotype: IgG1 Protein Concentration: 1 mg/ml Source: Rabbit Specificity: 5-hydroxymethylcytosine in single-stranded DNA from any mammalian, plant, insect, or microbial sources, as well as artificial templates used for standardization. Storage: Store at -20°C with prolonged storage at -80° C. Avoid repeated freeze/thawing.Shipping Weight: 0.125 lbsShipping Dimensions: 1.80 x 0.40 x 0.40Research or further manufacturing use only, not for food or drug use.
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